| Comparison of CDC assay with WHO standard method for detecting susceptibility levels of filariasis vector, Culex pipiens to some larvicides in Egypt |
| Paper ID : 1010-ISCBAS |
| Authors |
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Alaa Ismail Mohamed * Zoology and Entomology Dept, Faculty of Science, Al-Azhar University (girls branch), Cairo, Egypt |
| Abstract |
| Culex pipiens mosquito well known vector for the transmission of many vector-borne diseases. CDC was developed an alternative method to the WHO standard assay to detect resistance in mosquito adults and larvae within few hours. Culex pipiens were collected in larval stage from Qalubiya governorate and colonized with the laboratory population at the insectary of the Research Institute of Medical Entomology, Dokki, Giza, Egypt. The susceptibility assays were conducted on larval instars, against deflubenzron, chlorpyrifos and lambdacyhalthrin insecticides. The mortality percent, as well as the parameters of regression analysis, including LC50 and LC90, was calculated separately for the WHO and CDC methods. Both assays indicated that lambdacyhalthrin was the most effective insecticides followed by diflubenzuron and chlorpyrifos. Using CDC assay LC50 were 33-6, 2-4 and 7-4 ppm for lambdacyhalthrin, diflubenzuron and chlorpyrifos, respectively. While LC50 using WHO assay were; 43-6, 89-6 and 54-4 ppm for the three insecticides, respectively. The slopes obtained for both assays were almost like each other indicating the promising of the two assays. The lethal concentrations (LC50 and LC90) showed significant difference using both WHO and CDC methods. Both assays can be used in mosquito larvae to detect resistance but CDC one is more suitable for evaluating formulated insecticides in the field within a short time |
| Keywords |
| Susceptibility, Insecticide, WHO bioassay, CDC bioassay, Culex pipiens larvae |
| Status: Abstract Accepted (Poster Presentation) |