| Larvicidal activities of entomopathogenic nematode Steinernema carpocapsae and zinc oxide nanoparticles against the Black Cutworm, Agrotis ipsilon |
| Paper ID : 1030-ISCBAS |
| Authors |
|
Manal Elshaier *1, Wedad Atwa2, Mostafa Taha1, Afaf Abbas1, Asmaa Zu- Elhemma1, Hanaa Mohamed1, Abdelbaset Zayed1, walaa Ahmed Moselhy3, Halaa Kadada1, Fatma Hamed1, Hend Helmy1, Eman Gommaa1, Enayat Elqady1, Samia El- didamony1, Shaimaa Mohammed4 1Faculty of Science - Al-Azhar University 2Faculty of science - Al- Azhar University 3professor at Zoology and Entomology department 4Faculty of Science- Al- Azhar University |
| Abstract |
| The present study was conducted to determine the effect of entomopathogenic nematode Steinernema carpocapsae (S. carpocapsa) infective juveniles and zinc oxide nanoparticles (ZnO NPs) against Agrotis ipsilon larvae. Fourth larval instar was fed on castor leaves treated with different concentrations of S. carpocapsae (20, 40, and 80 IJs/larva) and ZnO NPs (150, 300, and 450ppm). Further biochemical assays and some histological studies were carried out to explain the effect of tested larvicides on A. ipsilon. The mortality percent of 4th larval instar due to the S. carpocapsae and ZnO NPs treatment increased up to 86.67 and 96.67%, respectively after 96 hours of application. The median lethal concentration (LC50) of S. carpocapsa IJs were 66.93, 38.71, and 25.86ppm at 48, 72, 96h PT, respectively. The corresponding LC50 values of ZnO NPs were 773.42, 396.70, 273.58, and 182.46ppm at 24, 48, 72, 96h PT, respectively. The results of biochemical assays and histological examination proved the lethal effects of the tested items. |
| Keywords |
| Agrotis ipsilon, control, entomopathogenic nematode, Steinernema carpocapsae, and zinc oxide nanoparticles |
| Status: Abstract Accepted (Poster Presentation) |