| Biochemical and toxicological effects of certain bioinsecticides on The Egyptian cotton leafworm Spodoptera littoralis |
| Paper ID : 1048-ISCBAS |
| Authors |
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Hend Helmy Salem * Lecturer of insect physiology, Zoology and Entomology Department, Faculty of Science, Al-Azhar University. |
| Abstract |
| The toxicity of five bioinsecticides on the Egyptian cotton leafworm (Spodoptera littoralis) were evaluated under laboratory conditions at 25 ±2Co, 65± 5R.H. and 14:10 (L.D.) photo phase. These bioinsecticides were Metarhizium anisopliae (Bio-Meta 2.5% WP), Beauveria bassiana (Bio-Ciana 2.5%WP), Bacillus thuringiensis (Protecto 9.4% WP), Emamectin benzoate (Excellent 1.9%WP) and Spinosad (Tracer 24%SC). All experiments were evaluated on 4th larval instar to determine the LC values of the tested compounds. The biochemical parameters were also recorded on the 4th larval instar after 72h. The LC50 of these compounds were 30.45, 38.61, 21.15, 7,98 and 9.22 ppm after 72h for the above-mentioned compounds, respectively. The results clearly indicated that Excellent was the most effective compound, while Bio-Ciana was the least effective compound. Generally, all treatments reduced total protein, total lipids, glucose contents, alkaline phosphatase (AKP), and acetylcholinesterase (AChE) significantly (P < 0.05) in comparison with the untreated control, while acid phosphatase (ACP) activity was decreased only after Bio-Meta, Excellent and Tracer treatments. On the other hand, Glutamic pyruvic transaminase (GPT) activity showed a significant increase (P< 0.05) with all treatments except Bio-Meta. Similarly, Glutamic oxaloacetic transaminase (GOT) activity was increased with all treatments except Bio-Meta and Bio-Ciana. We conclude that Excellent and Tracer were the most effective and can be successfully developed for the control of the Egyptian cotton leafworm. |
| Keywords |
| The Egyptian cotton leafworm, toxicity, bioinsecticides, biochemical parameters. |
| Status: Abstract Accepted |