| Evaluation of Biochemical Changes in Induced Diabetic Male Rats by Different Methods. |
| Paper ID : 1068-ISCBAS |
| Authors |
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Ahkam Mahmoud El- Gendy *1, Nageh Mabrouk Gaber2, Amira Mersal3, Omnia Mohy3 1Zoology and Entomology Dep. Al-Azhar University, Cairo,Egypt. 2Department of Medical Physiology, Faculty of Medicine (Boys), Al-Azhar University 3Zoology and Entomology Department, Faculty of Science (Girls), Al-Azhar University |
| Abstract |
| In Egypt, according to International Diabetes Federation, 2019, there were 8.9 million Egyptians suffering from diabetes in 2019. Different models are used to induce diabetes in different animals. This study aimed to evaluate the biochemical changes due to induction of diabetes in rats with different methods. Methods: male albino rats were used in this study. First group injected with Streptozotocin (STZ) 60mg/kg b.w, second group with150 mg alloxan/ kg b.w. The third group fed high fat diet (HDF, 30 %) for 2 months and the forth group (HF) drank 20% fructose solution for 2 months. Results: Both STZ and alloxan induced T1DM which was characterized by very high elevation in the blood glucose (405.20±64.13 and 424.40±32.61 mg/dl) in both groups respectively vs control group (86.70±4.57 mg/dl). Also, high decreases of insulin level were recorded in the same groups. HFD and HF groups revealed the presence of T2DM in these rats. Both blood glucose (104.20±11.00 and 104.30±10.36 mg/dl) and insulin levels were increased respectively. Many changes of both liver and kidney functions tests were altered. All groups had increased oxidative stress marker (MDA) which was associated with decreased antioxidants markers. Conclusion: the used models for diabetes induction can be used for assessment the treating trials of diabetes by drugs and medicinal plants. |
| Keywords |
| diabetes, streptozotocin, alalloxan, HFD, HF, biochemical parameters and oxidative stress. |
| Status: Abstract Accepted (Oral Presentation) |